Deborah A. Siegele
	Rank	Associate Professor
	Department	Biology
	Phone	(979) 862-4022
	Fax	(979) 845-2891
	Email Address	d-siegele@tamu.edu

Bacterial Stress Responses
My laboratory is studying how bacteria sense and adapt to stress in their environment, particularly low pH and nutrient depletion. We are using the model organism Escherichia coli for our studies. E. coli is ideal for this work because this Gram-negative bacterium lives in a wide variety of environments. E. coli can grow at temperatures between 10° and 44°C, it can grow when the external pH is between 5 and 9, and can survive prolonged periods of starvation. There are currently two projects going on in the lab: we are asking how environmental factors such as exposure to short chain fatty acids increases E. coli’s resistance to low pH, focusing on the signal transduction pathway involved in this process; and we are trying to determine the function of gene products that are needed for acid resistance.

1. Typas A, Nichols RJ, Siegele DA, Shales M, Collins SR, Lim B, Braberg H, Yamamoto N, Takeuchi R, Wanner BL, Mori H, Weissman JS, Krogan NJ & Gross CA (2008) High-throughput, quantitative analyses of genetic interactions in E. coli. Nat Methods
   
2. Hu JC, Aramayo R, Bolser D, Conway T, Elsik CG, Gribskov M, Kelder T, Kihara D, Knight TF Jr, Pico AR, Siegele DA, Wanner BL & Welch RD (2008) The emerging world of wikis. Science 320: 1289-90
   
3. Siegele DA (2005) Universal stress proteins in Escherichia coli. J Bacteriol 187: 6253-4
   
4. Ganesh R, Siegele DA & Ioerger TR (2003) MOPAC: motif finding by preprocessing and agglomerative clustering from microarrays. Pac Symp Biocomput 41-52
   
5. Champion MM, Campbell CS, Siegele DA, Russell DH & Hu JC (2003) Proteome analysis of Escherichia coli K-12 by two-dimensional native-state chromatography and MALDI-MS. Mol Microbiol 47: 383-96
   
6. Arnold CN, McElhanon J, Lee A, Leonhart R & Siegele DA (2001) Global analysis of Escherichia coli gene expression during the acetate-induced acid tolerance response. J Bacteriol 183: 2178-86
   
7. Siegele DA, Campbell L & Hu JC (2000) Green fluorescent protein as a reporter of transcriptional activity in a prokaryotic system. Methods Enzymol 305: 499-513
   
8. Mao W & Siegele DA (1998) Genetic analysis of the stationary phase-induced mcb operon promoter in Escherichia coli. Mol Microbiol 27: 415-24
   
9. Siegele DA & Hu JC (1997) Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci U S A 94: 8168-72
   
10. Siegele DA & Guynn LJ (1996) Escherichia coli proteins synthesized during recovery from starvation. J Bacteriol 178: 6352-6
    
11. Siegele DA, Imlay KR & Imlay JA (1996) The stationary-phase-exit defect of cydC (surB) mutants is due to the lack of a functional terminal cytochrome oxidase. J Bacteriol 178: 6091-6
    
12. Siegele DA & Kolter R (1993) Isolation and characterization of an Escherichia coli mutant defective in resuming growth after starvation. Genes Dev 7: 2629-40
    
13. Zambrano MM, Siegele DA, AlmirГіn M, Tormo A & Kolter R (1993) Microbial competition: Escherichia coli mutants that take over stationary phase cultures. Science 259: 1757-60
    
14. Kolter R, Siegele DA & Tormo A (1993) The stationary phase of the bacterial life cycle. Annu Rev Microbiol 47: 855-74
    
15. Marquardt JL, Siegele DA, Kolter R & Walsh CT (1992) Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase. J Bacteriol 174: 5748-52
    
16. Dombroski AJ, Walter WA, Record MT Jr, Siegele DA & Gross CA (1992) Polypeptides containing highly conserved regions of transcription initiation factor sigma 70 exhibit specificity of binding to promoter DNA. Cell 70: 501-12
    
17. Siegele DA & Kolter R (1992) Life after log. J Bacteriol 174: 345-8
    
18. Siegele DA, Hu JC, Walter WA & Gross CA (1989) Altered promoter recognition by mutant forms of the sigma 70 subunit of Escherichia coli RNA polymerase. J Mol Biol 206: 591-603
    
19. Siegele DA, Hu JC & Gross CA (1988) Mutations in rpoD, the gene encoding the sigma 70 subunit of Escherichia coli RNA polymerase, that increase expression of the lac operon in the absence of CAP-cAMP. J Mol Biol 203: 29-37
    
20. Frackman S, Siegele DA & Feiss M (1985) The terminase of bacteriophage lambda. Functional domains for cosB binding and multimer assembly. J Mol Biol 183: 225-38
    

Publications list from Pubmed